User:Anthony Salvagno/Notebook/Research/2009/05/08/Not Ligations

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So yesterday I ligated the anchor to the hairpin adapter, and then digested the hairpin with NotI. Today I PCR purified (QIAQuick) the digested sample because the hairpin fragment would just go through the membrane of the column. Then I ran a ligation of this construct to the Notsamples.

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Next time I will gel purify these guys.