User:Anthony Salvagno/Notebook/Research/2009/04/21/BstXI Digested Gel Extraction

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I came in early this morning (because Stef has an 8am class... I mean because I couldn't wait to get back to work) and picked up where I left off on Friday. Remember:

  1. PCR-ed the anchor segment of DNA.
  2. Digested the PCR-ed segment with BstXI (so there is now a sticky overhang)

This morning I setup a gel so that the little 40bp segment of DNA from the BstXI digestion separates from the rest of the anchor. I then gel extract the piece I want (should be the only visible piece), then I wait for my oligos to arrive so I can do the next part.