User:Anthony Salvagno/Notebook/Research/2009/02/24/gDNA digestion try 2

I remembered doing this stuff, but forgot to write it up since it was such a short day at Osley Lab. Anyways. Here is what I did in quick:

1. prepared:
   • 24ul gDNA
   • 7.5ul NEB Buffer 2
   • 7.5ul 10x BSA
   • 4uL XhoI
   • 32uL H₂O
2. total of 75uL
3. after 2 hours I added 3uL more XhoI
4. waited another hour or something
5. EtOH extraction
6. This cocktail gave us 42.6ng/uL gDNA after digestion

I also have (in my notebook 1.0) 22ng/uL of pRS DNA. That is probably from the gel extraction.