User:Anthony Salvagno/Notebook/Research/2009/02/18/Plasmid DNA from Clones

Protocol

We are following the protocol from a kit so I won't bother to write down the instructions. The kit is from QIAGEN and is taken from the QIAprep Miniprep Handbook. The Protocol is called: "Plasmid DNA purification using the QIAprep spin Miniprep Kit and Microcentrifuge." It can be found on page 22 of the handbook.

Each test tube is a different colony of E. coli cells which represents a single clone. The technique is important for later experiments where having individual clones per test tube are important.

Results

Nanodrop reading = 149ng/uL

Comments

So until literally just now I really had no clue what I was doing. I knew the words plasmid, cloning, and prep all belonged in there, but I didn't really know in what order. I officially can explain what I did for the past couple of days. Here it goes:

We inserted plasmid DNA that Cory made into E. coli for cloning. We cloned that DNA over a couple of nights (1st to create colonies with the insert, then to grow individual colonies to extract the DNA). Today I extracted the DNA from the clones and measured our concentration. I then gave Cory back her original plasmid DNA. That sums it up.