User:Anthony Salvagno/Notebook/Research/2009/02/05/Measuring OD
The biologists employ the use of a spectrometer to measure how many cells are in solution.
The device works off a control to measure all other samples. In my experiments the control has been an empty solution.
- YPD for yeast culture
- 1% SDS with 2-ME (mercaptoethanol) when working with the lyticase
- Press any key to begin
- load samples with "window" of sample chamber aligned with hole in holder
- set program and number of samples
- run experiment
- collect data
I've yet to figure out the nomenclature of the setup. To me we just get some unitless number (perhaps this is true) and we ultimately want a smaller (or sometimes greater) number. Then we use that number and manipulate it to get the concentration of "particles" that we want. For an example please see User:Anthony Salvagno/Notebook/Research/Yeast_Genomic_DNA_Prep and User:Anthony Salvagno/Notebook/Research/2009/02/05/Spheroplasting Try 1.