User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/11/15

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Sample Prep

Solutions for UV-Vis analysis as shown:

Solutions were prepared with 53.1 mM Fructose, 3.45 mM gold, and 34.3 mM silver. All other stock solutions were as stated in the tables.

Silver A Anneliesefaustino161109.PNG

Silver B redo actually correct this time amf 161109.PNG

Silver C amfaustino 161109.PNG

Gold pH 11 rerunanneliesefaustino 161109.PNG

Data Analysis

Ag0.5Amf.PNG

This chart shows the absorbance spectra of 0.5 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. The maximums appear to be around 420 nm. pH 8 appears to have the strongest absorbance, while pHs 11 and 12 appear to have the lowest around 420 nm. pH 11 and 12 have their maximums shifted right from the rest of the data.

Ag0.5Amfa530.PNG

This chart shows the A(530) for 0.5 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs.


Ag0.0625 AMF.PNG

This chart shows the absorbance spectra of 0.0625 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. pH 11 shows a massive peak with an absorbance of almost 2. An absorbance this high has never been observed in any of our other trials of gold or silver. This is particularly odd because of the low concentration of fructose used and the high pH of the sample.

Ag0.0625a530amf.PNG

This chart shows the A(530) for 0.0625 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs.


Ag1.25amf.PNG

This chart shows the absorbance spectra of 1.25 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs. The maximums appear to be around 420 nm. pH 8 appears to have the strongest absorbance, while pHs 11 and 12 appear to have the lowest around 420 nm. pH 11 and 12 have their maximums shifted right from the rest of the data just as in 0.5 mM fructose. pH 12 is of particular interest because it shows a peak around 270 nm. None of the other samples exhibit this peak.

Ag1.25amfa530.PNG

This chart shows the A(530) for 1.25 mM Fructose with no BSA and 0.25 mM Ag NP at varied pHs.

AupH11varied.PNG

This chart shows the UV-Vis absorption spectra for pH 11 samples with varied concentrations of fructose and 0.25mM gold NPs. The graphs are fairly consistent except for 0.75 mM Fructose, which has a slightly lower absorption maximum.

AupH11varieda530amf.PNG

This chart shows the a(530) for pH 11 samples with varied concentrations of fructose and 0.25mM gold NPs.

15203275 1293018170771473 6884921347448013591 n AMFAUSTINO.jpg

This image shows the pH 11 samples of Au NPs with varied concentrations of fructose. From left to right: 0.0625, 0.125, 0.25, 0.75, and 1.25 mM Fructose.

15253448 1293018197438137 1037747955419605573 n AMFAUSTINOOOO.jpg

This image shows the two samples that produced interesting results. On the left is pH 12 Ag with 1.25 mM Fructose. This sample had an interesting peak aruond 270 nm. On the left is Ag 0.0625 mM Fructose pH 11. This sample had a very high absorbance, which is visually confirmed by the intensity of the yellow color.