User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/11/08

Project name

Main project page Previous entry      Next entry

Sample Preparation BSA Ocean Optics pH 10 [Au]=0.25 mM [BSA]=3.125 uM Vf=3000 uL Stock Concentrations 161108 [BSA]= 55.1 uM [Au]= 3.45 mM [NaOH]= 1 mM Volumes for making the solution: 170.15 uL BSA 217.39 uL Au 2312.46 uL HOH 300 uL NaOH Data Analysis The plot of maximum absorbance over time for pH 10, [BSA]=3.125 uM, [Au]=0.25 mM is shown above. In this chart, we see a steady increase in maximum absorbance over time. At this high pH, it took a much longer time for nanoparticles to form than in previous runs with more acidic pHs. We expect for the next ocean optics run to not form nanoparticles (based off of our UV-Vis data). The plot of maximum wavelength over time for pH 10, [BSA]=3.125 uM, [Au]=0.25 mM is shown above. In this plot, we see the maximum wavelength hold around 480/490 nm from 0s until 3800s. This plateau region has more fluctuations than some of our other less acidic runs. At around 3800s, the maximum wavelength rapidly increases to 550 nm and slowly decreases for the rest of the run to about 530 nm. The plot of (A535.15) over time for pH 10, [BSA]=3.125 uM, [Au]=0.25 mM is shown above. In this plot, we see a steady increase in absorbance over time. This increase seems to accelerate around 3800s, which is reasonable with the data from the maximum wavelength over time plot.