User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/11/08

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Sample Preparation

BSA Ocean Optics

  1. pH 10
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 161108

  1. [BSA]= 55.1 uM
  2. [Au]= 3.45 mM
  3. [NaOH]= 1 mM

Volumes for making the solution:

  1. 170.15 uL BSA
  2. 217.39 uL Au
  3. 2312.46 uL HOH
  4. 300 uL NaOH

Data Analysis

Max abs over time pH 10 amf 161109.PNG

The plot of maximum absorbance over time for pH 10, [BSA]=3.125 uM, [Au]=0.25 mM is shown above. In this chart, we see a steady increase in maximum absorbance over time. At this high pH, it took a much longer time for nanoparticles to form than in previous runs with more acidic pHs. We expect for the next ocean optics run to not form nanoparticles (based off of our UV-Vis data).

Max wavlength time amf 161109.PNG

The plot of maximum wavelength over time for pH 10, [BSA]=3.125 uM, [Au]=0.25 mM is shown above. In this plot, we see the maximum wavelength hold around 480/490 nm from 0s until 3800s. This plateau region has more fluctuations than some of our other less acidic runs. At around 3800s, the maximum wavelength rapidly increases to 550 nm and slowly decreases for the rest of the run to about 530 nm.

A535.15 amf ocean optics 161109.PNG

The plot of (A535.15) over time for pH 10, [BSA]=3.125 uM, [Au]=0.25 mM is shown above. In this plot, we see a steady increase in absorbance over time. This increase seems to accelerate around 3800s, which is reasonable with the data from the maximum wavelength over time plot.