User:Anh Do/Notebook/CHEM 481 Polymer/2017/02/01

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Task list Prepare beads with 10%, 25% and 40% clay content Make bentonite solution Bentonite 3wt% solution Mass of bentonite powder: 3.00014 g Volume of water: 97 mL Synthesize beads - 10% clay content Create a solution of PVOH, bentonite solution, water, and HCl. 1.25g PVOH, 5 mL bentonite solution, 15mL HCl, 35mL water Place this solution on a hot plate and heat/stir until everything has dissolved (for 30 mins). Add 30 mL of this solution to a 100 mL solution of ethyl acetate in a dropwise manner stirring at 380 rpm. Add 1.5 mL of glutaraldehyde and stir at 260 rpm for 3 minutes. Add 1.5 mL of glutaraldehyde and stir at 260 rpm for 7 minutes. Put the solution and the beads to two 50mL falcon tubes. Centrifuge the falcon tube at 3000rpm once for one minute. Remove the solution in the falcon tube and add in water. Synthesize beads - 25% clay content Create a solution of PVOH, bentonite solution, water, and HCl. 1.25g PVOH, 13 mL bentonite solution, 15mL HCl, 27mL water Place this solution on a hot plate and heat/stir until everything has dissolved (for 30 mins). Add 30 mL of this solution to a 100 mL solution of ethyl acetate in a dropwise manner stirring at 380 rpm. Add 1.5 mL of glutaraldehyde and stir at 260 rpm for 3 minutes. Add 1.5 mL of glutaraldehyde and stir at 260 rpm for 7 minutes. Put the solution and the beads to two 50mL falcon tubes. Centrifuge the falcon tube at 3000rpm once for one minute. Remove the solution in the falcon tube and add in water. Notes Solution in burrette tends to stop flowing through every 5 - 7mL. It should takes approximately 20 seconds for 1mL to flow through otherwise. For the beads with 10% clay content, we used less than 3mL of glutaraldehyde since there wasn't enough solution left in the bottle.