User:Anh Do/Notebook/CHEM 481 Polymer/2016/10/07

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UV Vis - Testing Beads (cont.)

  • Continue with the previous procedure for the 20% and 30% beads
    • Start using a pump to suck out water from column because the beads are too fine for water to flow through quickly enough.
      • Clean the right end of the pumping tube by letting it pump out clean water (This tube usually has soap inside)
      • Connect the pumping tube directly to the column (Figure 1)
      • Fill the tube with 5 ml of MG 50 ppm
      • Place the left end of the pumping tube at the rim of a vial for collection
      • Change the speed to 15 rpm and press start
      • Press stop when the solution in the column is totally pumped out (it usually takes minutes)
      • Label the vial