User:Andrew K Farag/Notebook/CHEM-581/2014/08/29

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Objective

Completing film synthesis, starting new film synthesis, and making stock Malachite green solutions.

Description

  1. Completing film synthesis
    1. Warm 150mL of Sodium Sulfate Solution (see below for details) at 80C for 15 minutes
    2. Place film in Sodium Sulfate Solution (see below for details) at 80C for 1 hour with gentle stirring
    3. Warm 150mL of 1% HCl at 80C for 15 minutes
    4. Place film in 1% HCl Solution (see below for details) at 80C for 30 minutes with gentle stirring
    5. Place film in Sodium Bicarbonate Solution for 15 minutes at room temperature with gentle stirring
    6. store in water
  2. Starting new film synthesis
    1. Use Dr. Hartings' Wednesday's Procedure to make new 10% clay-polyvinyl alcohol film
      1. It was 10% by weight clay because we used 1g of PVA and 0.11g of clay
  • PVA weight-1.0029g (Polymer only)
  • PVA weight-1.0038g (Polymer and clay)
  • Na montmorillonite-0.1105g
  1. Malachite Green Solutions
    1. We need to make the following solutions
      1. 25mL 0.20ppm Malachite Green
      2. 25mL 0.50ppm Malachite Green
      3. 25mL 1.1ppm Malachite Green
      4. 25mL 1.4ppm Malachite Green
      5. 25mL 1.7ppm Malachite Green
      6. 50mL 2.0ppm Malachite Green
      7. 50mL 8.0ppm Malachite Green
      8. 50mL 80.0ppm Malachite Green
      9. 50mL 200.0ppm Malachite Green
        1. My group prepared the solutions for the 0.20ppm and 0.50ppm Malachite Green from a 200ppm stock solution. The rest of the solutions were prepared by other groups in the lab section.
    2. Take a UV-Visible spectrum of the 0.2ppm to 8ppm standards.
    3. Make a standard curve to determine the molar absorptivity of Malachite Green
  2. Two sets are to be prepared; each set includes a different kind of film (Polymer and Polymer+Clay). The weights of the films should be recorded as well.

Films.in.vials.png

Data

Stock Solutions

  1. 0.2M Sodium Sulfate Solution
    1. I dissolved 22.034g of anhydrous Na2SO4 in 1000mL of water
    2. I added 100mL of concentrated H2SO4 to this solution
    3. This solution will be used to enable the reaction between polyvinyl alcohol and glutaraldehyde in order to cross link the polymer film
  2. 1% wt. HCl Solution
    1. I added 10mL of concentrated HCl to 985mL of water
    2. This solution will be used to enable the reaction between polyvinyl alcohol and glutaraldehyde in order to cross link the polymer film
  3. 0.2M Sodium Bicarbonate Solution
    1. I dissolved 17.042g of NaHCO3 in 1000mL of water
    2. This solution will be used to neutralize the acid in your films

The Making of Malachite Green Stock Solutions

We prepared the 0.20 ppm and 0.50 ppm Malachite Green Stock Solutions.

Melvin, Becky, and Eleni prepared the 1.1 ppm and 1.4 ppm Malachite Green Stock Solutions.

Madeleine and Alicia prepared the 1.7 ppm and 2.0 ppm Malachite Green Stock Solutions. See Madeleine's 8/29 Page for their calculations.

James, Jake, and Mary prepared the 8.0 ppm, 80.0 ppm, and 200.0 ppm Malachite Green Stock Solutions. See James's 8/29 Page for their calculations.

      1. 25mL 0.20ppm Malachite Green - made with 0.025mL of 200.0ppm solution in 24.975mL water
      2. 25mL 0.50ppm Malachite Green - made with 0.0625mL of 200.0ppm solution in 24.9375mL water
      3. 25mL 1.1ppm Malachite Green - made with
      4. 25mL 1.4ppm Malachite Green - made with
      5. 25mL 1.7ppm Malachite Green - made with 5.31mL of 8.0ppm solution in 19.69mL water
      6. 100mL 2.0ppm Malachite Green - made with 1mL of 200.0ppm solution in 99.0mL water
      7. 50mL 8.0ppm Malachite Green - made with 2mL of 200.0 ppm solution in 48.0mL water
      8. 50mL 80.0ppm Malachite Green - made with 20mL of 200.0ppm solution in 30.0 mL water
      9. 50mL 200.0ppm Malachite Green - made with 20.00mg of MG in 100mL volumetric flask.

Calculations

Our calculations for the dilution of the stock solution to solutions 25 mL of 0.20 ppm and 0.50 ppm are as follows:

Conc1*V1 = Conc2*V2

  • .20ppm
    • (.20ppm)(25mL)=(200.0ppm)*V
    • V=0.025mL of 200.0ppm solution in 24.975mL water
  • 0.5ppm
    • (0.5ppm)(25mL)=(200ppm)*V
    • V=0.0625mL of 200ppm solution in 24.9375mL water