User:Andrew Barney/Notebook/Old Fruit Fly Lab/2007/10/11

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Lab 2

Problem: How can we determine the Mode of Inheritance of a mutant trait in fruit flies?

Information: We know...

a. there are four basic modes of inheritance. AR, AD, XLD, XLR. (Autosomal Recessive, Autosomal Dominant, X-Linked Dominant, and X-Linked Recessive)

b. our mutant fruit flies are caused by a single mutation (1 gene).

c. mendel's laws and how to analyze simple crosses.

d. our wild fruit flies are inbred for homozygous.

e. how to raise fruit flies and how to collect virgin females.

  • Observations: 10/11 - Today we put 8 male wilds and put them with 9 or 10 female sepia into vial #1. Then 8 male sepia with 8 female wild into vial #2.
  • Hypothesis: If we perform reciprocal crosses on our fruit flies, we will be able to determine the Mode of Inheritance of the sepia trait.

8 wild female X 8 males sepia
9 or 10 sepia female X 8 males wild

  • Observation: 10/11/07 - Thursday - We performed reciprocal crosses today between the sepia and wild flies.

Experimental Design

We will set up two genetic crosses. Vial 1 = Male wild X Female sepia Vial 2 = Female wild X Male sepia


Expected results Vial 1 Vial 2
AR 100% Wild 100% Wild
AD 100% Sepia 100% Sepia
SLR All male sepia, All female wild 100% Wild
SLD 100% Sepia All female sepia, All male wild

Punnett Squares

  • I forgot to include the Punnett Squares in my original lab, but they are pretty important. Try not to forget these when you record your data!

AR r r
R 100% Wild 100% Wild
R 100% Wild 100% Wild

r 100% Sepia 100% Sepia
r 100% Sepia 100% Sepia

SLR (sepia females) x x
X wild wild
Y sepia sepia

SLR (sepia males) X X
x wild wild
Y wild wild

SLD (sepia females) X X
x sepia sepia
Y sepia sepia

SLD (sepia males) x x
X sepia sepia
Y wild wild