User:Allison K. Alix/Notebook/CHEM-581/2013/01/30

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Begin hydrogel formation using freeze-thaw method for the design of a system that allows for the oral delivery of insulin.


Currently the oral delivery of insulin is not possible due to it's degradation in the stomach before reaching the bloodstream. This project will employ a system comprised of a hydrogel core in which insulin will be distributed(to begin synthesis today) which will ultimately be coated by a lipid bilayer. The purpose for the lipid bilayer is for the system to successfully pass through the stomach acid (lipids are not digested until reaching the small intestine) at which point the hydrogel will be exposed and the insulin will be absorbed into the blood.


1) Dissolve 0.5g PVOH of the following molecular weights in 6mL of H2O @ 95°C :

a) 22,000 (MP Biomedicals)

b) 89,000-98,000 99+% hydrolysis(Aldrich Chemistry)

c) 130,000 99+% hydrolysis(Aldrich Chemistry)

d) 146,000-186,000 99+% hydrolysis(Aldrich Chemistry)

Make 2 samples of each different Molecular Weight.

2) Prepare 4 more films, one of each MW, with the addition of a dye, Rhodamine-G-6 prior to casting.

3) Place all prepared films in a freezer at -20°C for 24 hours. Remove and thaw for 24 hours. Repeat this cycle for a total of 3 freeze-thaw cycles.


Preparation of 1mM R6G

molar mass R6G = 479.02 g/mol

479.0 g/mol x 1mmol/1L x 1mol/1000mmol x 10mL x 1L/1000mL = 0.00479g in 10mL ethanol