User:Allison K. Alix/Notebook/CHEM-581/2012/10/24

Experimental Chemistry

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Objective Take UV-Vis of porphyrin solutions Re-do crosslinking of porphyrin films Prepare sulfate solutions Description UV-Vis Spectra of porphyrin solutions with the following concentrations were collected: 25μM 12.5μM 6.75μM 3.375μM 1.6875μM 10 mL 50 μM of the following sulfate solutions were prepared: Na2SO4 Fe2(SO4)3 Al2(SO4)3 Data Preparation of 50 μM Na2SO4 [math]\displaystyle{ \frac {142.07 g} {mol} \times \frac {50mmol} {L} \times \frac {10^-3 mol} {1mmol} \times \frac {1 L} {1000mL} \times 10mL = 0.071035 g }[/math] 0.071035 g Na2SO4 in 10mL H2O to prepare 50mM. Dilute to 50μM by using 10μL 50mM in 9.99mL H2O. Preparation of 50 μM 72 wt% Fe2(SO4)3 [math]\displaystyle{ \frac {72} {100} \times \frac {399.88 g} {mol} \times \frac {50mmol} {L} \times \frac {10^-3 mol} {1mmol} \times \frac {1L} {1000mL} \times \frac 10mL = 0.144g }[/math] 0.144 g 72 wt% Fe2(SO4)3 in 10mL H2O to prepare 50mM. Dilute to 50μM by using 10μL 50mM in 9.99mL H2O. Preparation of 50 μM Al2(SO4)3 [math]\displaystyle{ \frac {656.33 g} {mol} \times \frac {50mmol} {L} \times \frac {10^-3 mol} {1mmol} \times \frac {1L} {1000mL} \times 10mL = 0.328g }[/math] 0.328 g Al2(SO4)3 in 10mL H2O to prepare 50mM. Dilute to 50μM by using 10μL 50mM in 9.99mL H2O. Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.