User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/15

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Nuclear Region Adenita Culture 454 Amplification

Adenita Cultures 41-44

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water 5.15 25.75
Takara EX Buffer 10X 1 5
dNTP 2.5uM 0.8 4
Primer Mix 20uM 1
EX Taq 5U 0.05 0.25
Template Instagene 2 10
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C 10 sec
60C 1 min
x35
AMP Lane Template Primer AMP Lane Template Primer
1 41 Instagene B5 16 44 Instagene B8
2 41 Instagene C5 17 44 Instagene C8
3 41 Instagene D5 18 44 Instagene D8
4 41 Instagene E5 19 44 Instagene E8
5 41 Instagene F5 20 44 Instagene F8
6 42 Instagene B6 21 Neg Control B5
7 42 Instagene C6 22 Neg Control C5
8 42 Instagene D6 23 Neg Control D5
9 42 Instagene E6 24 Neg Control E5
10 42 Instagene F6 25 Neg Control F5
11 43 Instagene B7 26 Pos Control B5
12 43 Instagene C7 27 Pos Control C5
13 43 Instagene D7 28 Pos Control D5
14 43 Instagene E7 29 Pos Control E5
15 43 Instagene F7 30 Pos Control F5
*Positive Control = AV 20ng

Load 2.0ul Sample

15May10A.jpg

Nuclear Region Adenita Culture 454 Amplification Status

CULTURE PRIMER COLUMN GOOD REDO? REDO Result
Instagene 1 B1-F1 1 Y 10May
Instagene 2 B2-F2 1 Y 10May
Instagene 3 B3-F3 1 Y 11May
Instagene 4 B4-F4 1 Y 11May
Instagene 5 B5-F5 1 Y 11May
Instagene 6 B6-F6 1 Y 11May
Instagene 7 B7-F7 1 Y 12May
Instagene 8 B8-F8 1 Y 12May
Instagene 9 B9-F9 1 Y 12May
Instagene 10 B10-F10 1 B10, C10, F10 weak 12May
Instagene 11 B11-F11 1 X Failed
Instagene 12 B12-F12 1 Y 12 May
Instagene 13 B1-F1 2 WEAK 12May
Instagene 14 B2-F2 2 Y 12 May
Instagene 15 B3-F3 2 Y 12 May
Instagene 16 B4-F4 2 Y 12 May
Instagene 17 B5-F5 2 D5, E5, F5 weak 12May B5, C5, D5, E5, F5 13May
Instagene 18 B6-F6 2 X Failed
Instagene 19 B7-F7 2 D7, E7, F7 weak 12May D7 13May
Instagene 20 B8-F8 2 C8
Instagene 21 B9-F9 2 X
Instagene 22 B10-F10 2 y 14May D10, E10 12May
Instagene 23 B11-F11 2 y 14May C11, D11, E11, F11 12May
Instagene 24 B12-F12 2 y 14May X
Instagene 25 B1-F1 3 y 14May D1 13May
Instagene 26 B2-F2 3 Y 13May
Instagene 27 B3-F3 3 D6 weak 13May failed 14May
Instagene 28 B4-F4 3 B7, C7 13May only C4 14May
Instagene 29 B5-F5 3 C5 13 May C5 14May
Instagene 30 B6-F6 3 Y 14May B6, C6, D6 13 May
Instagene 31 B7-F7 3 X B7, C7, E7 14May
Instagene 32 B8-F8 3 X failed
Instagene 33 B9-F9 3 X
Instagene 34 B10-F10 3 X
Instagene 35 B11-F11 3 X
Instagene 36 B12-F12 3 X
Instagene 37 B1-F1 4 x
Instagene 38 B2-F2 4 x
Instagene 39 B3-F3 4 x
Instagene 40 B4-F4 4 x
Instagene 41 B5-F5 4 x
Instagene 42 B6-F6 4 x
Instagene 43 B7-F7 4 x
Instagene 44 B8-F8 4 x D8, E8 15May
Instagene 45 B9-F9 4
Instagene 46 B10-F10 4
Instagene 47 B11-F11 4
Instagene 48 B12-F12 4
Instagene 49 B1-F1 5
Instagene 50 B2-F2 5
Instagene 51 B3-F3 5
Instagene 52 B4-F4 5
Instagene 53 B5-F5 5
Instagene 54 B6-F6 5
Instagene 55 B7-F7 5
Instagene 56 B8-F8 5
Instagene 57 B9-F9 5
Instagene 58 B10-F10 5
Instagene 59 B11-F11 5
Instagene 60 B12-F12 5
Instagene 61 B1-F1 6
Instagene 62 B2-F2 6
Instagene 63 B3-F3 6
Instagene 64 B4-F4 6
Instagene 65 B5-F5 6
Instagene 66 B6-F6 6
Instagene 67 B7-F7 6
Instagene 68 B8-F8 6
Instagene 69 B9-F9 6
Instagene 70 B10-F10 6
Instagene 71 B11-F11 6
Instagene 72 B12-F12 6
Instagene 73 B1-F1 7
Instagene 74 B2-F2 7
Instagene 75 B3-F3 7
Instagene 76 B4-F4 7
Instagene 77 B5-F5 7
Instagene 78 B6-F6 7
Instagene 79 B7-F7 7
Instagene 80 B8-F8 7
Instagene 81 B9-F9 7
Instagene 82 B10-F10 7
Instagene 83 B11-F11 7
Instagene 84 B12-F12 7
Instagene 85 B1-F1 8
Instagene 86 B2-F2 8