User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/02

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Alien genes Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Testing HCO/LCO on diverse rotifer DNA

Reagents 1x conc. 4
h2o 5.75 - 23
10x 1 10x 4
dye 1 10x 4
dNTP 0.2 10 mM 0.8
Forward 0.5 10 uM 2
Reverse 0.5 10 uM 2
Taq 0.05 5 U/um 0.2
DNA 1 50 ng/ul
TOTAL 10 Cocktail per vial 9
  • Program
3m 95C (10s 94C; 30s 50C; 30s 72C)x35
  • Species
H. constricta M. quad P. roseola P. rapida

Gid 2009-11-02 18hr 30min.jpg

  • Only Habrotrocha amplified. Maybe these primers are not going to be the best for large-scale genetic surveys.