User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/02

Alien genes

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Testing HCO/LCO on diverse rotifer DNA Reagents 1x conc. 4 h2o 5.75 - 23 10x 1 10x 4 dye 1 10x 4 dNTP 0.2 10 mM 0.8 Forward 0.5 10 uM 2 Reverse 0.5 10 uM 2 Taq 0.05 5 U/um 0.2 DNA 1 50 ng/ul TOTAL 10 Cocktail per vial 9 Program 3m 95C (10s 94C; 30s 50C; 30s 72C)x35 Species H. constricta M. quad P. roseola P. rapida Only Habrotrocha amplified. Maybe these primers are not going to be the best for large-scale genetic surveys.