User:Alexander S Mikheyev/Notebook/bee methylome/2010/04/13

Project name

Main project page Previous entry      Next entry

Library preparation ' Reaction mixture conc. Callow Mature Callow (after bisulfite conversion) ?? 2 3 PCR primer PE 1.0 ?? 0.2 0.2 PCR primer PE 2.0 ?? 0.2 0.2 Phusion DNA polymerase 2x 5 5 MilliQ 2.6 1.6 total 10 10 98C 30 sec 98C 10 sec 65C 30 sec 72C 30 sec 18 cycle 72C 5 min load 5.0 ul of sample ' Reaction mixture conc. Callow Mature Callow (after bisulfite conversion) ?? 2 3 PCR primer PE 1.0 ?? 0.2 0.2 PCR primer PE 2.0 ?? 0.2 0.2 Phusion DNA polymerase 2x 5 5 MilliQ 2.6 1.6 total 10 10 98C 30 sec 98C 10 sec 65C 30 sec 72C 30 sec 22 cycle 72C 5 min load 5.0 ul of sample