User:Alexander S Mikheyev/Notebook/bee methylome/2010/04/13

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Library preparation

'
Reaction mixture conc. Callow Mature
Callow (after bisulfite conversion) ?? 2 3
PCR primer PE 1.0 ?? 0.2 0.2
PCR primer PE 2.0 ?? 0.2 0.2
Phusion DNA polymerase 2x 5 5
MilliQ 2.6 1.6
total 10 10
98C 30 sec
98C 10 sec
65C 30 sec
72C 30 sec
18 cycle
72C 5 min

load 5.0 ul of sample

0074.jpg

'
Reaction mixture conc. Callow Mature
Callow (after bisulfite conversion) ?? 2 3
PCR primer PE 1.0 ?? 0.2 0.2
PCR primer PE 2.0 ?? 0.2 0.2
Phusion DNA polymerase 2x 5 5
MilliQ 2.6 1.6
total 10 10
98C 30 sec
98C 10 sec
65C 30 sec
72C 30 sec
22 cycle
72C 5 min

load 5.0 ul of sample

0075.jpg