User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/11/06

Biomaterials Design Lab

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Objective Synthesize more Lysozyme linked AuNP's and Hemoglobin linked AuNP's. Procedure Stock Solution Prep Gold- .011 grams was added to a 10 ml volumetric flask and was filled to 10 ml with water. Hemoglobin- .0032 grams of hemoglobin was added to a 10 ml volumetric flask and was filled to 10 ml with water. Lysozyme- .02 grams of lysozyme was added to a 25 ml volumetric flask and was filled to 25 ml with water. Specific concentrations are expressed below. Sample Prep AuNP's were made as dictated below. Once the solutions were mixed they were heated for 4 hours at 80 degrees Celsius. Data No data today! Note after the samples were made, all of the hemoglobin AUNP's were clear with fibers except for the smallest ratio that was pink. All of the lysozyme AuNP's made today were pink except ratios higher than 30:1 had fibers.