User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/11

Biomaterials Design Lab

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Objective To run atomic absorption spectra for gold solution standards as dictated by Dr.Hartings here.<br.> Form a calibration curve with the class data to use for future reference.<br.> Calculate Concentration of Gold in Citrate Au-NP's and BSA Au-NP's.<br.> Calculate the number of gold particles in each sample of Citrate Au-NP's and BSA Au-NP's.<br.> Procedure Samples were prepared as dictated by the table below.<br.> <br.> Please note that all samples were measured using a 100-microliter pipette and a 1000-microliter pipette.<br.> Data Individual and Group Calibration Curve The table below represents the results from the atomic absorption spectra. Please note that the max absorbance of gold was 248 nm.<br.> Also note that the Citrate Au-NP had to be diluted in order view on AAS. Further calculations must reflect this dilution.<br.> <br.> Absorbance had to be corrected in order to reflect the non-zero water absorbance. Note individual data is on the left and the class data is on the right. Grubbs Test Analysis of Group Data Based on the Grubbs test, and using a value of n=5 for each concentration, no data point had a Grubbs value greater than 1.67 using 95% confidence.<br.> Do to this fact none of the data points are outliers. Calculation of Concentration of Gold in Citrate Au-NP's and BSA Au-NP's Note that the best fit of the calibration curve is a polynomial.<br.> Using this best fit and the calculated absorbances of the Au-NP's the concentration of gold are the following,<br.> Concentration of Gold in Citrate Au-NP's = 74.45 ug/ml (This takes into account the dilution). <br.> Concentration of Gold in BSA Au-NP's = 38.35 ug/ml <br.> Calculation of the # of Gold Particles in each Au-NP Volume of BSA Au-NP = 2.809ml Using the concentrations calculated using the AAS the amount of gold in the whole solution is, .00000106 moles. Using avogadros number their is, 6.383 * 10E17 atoms of gold in the BSA Au-NP solution Volume of Citrate Au-NP = 25.6 ml Using the concentrations calculated using the AAS the amount of gold in the whole solution is, .00000498 moles Using avogadros number their is, 2.999 * 10E18 atoms of gold in the citrate Au-NP solution. Know the concentration of the nanoparticles in solution is 8.43 nM