UCLAiGEM:Notebook/MaterialsProcessing/2015/05/05

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<html><head><title>5 May</title><meta content="text/html; charset=UTF-8" http-equiv="content-type"><style type="text/css">ul.lst-kix_i14bgtn5okxt-1{list-style-type:none}ul.lst-kix_i14bgtn5okxt-2{list-style-type:none}.lst-kix_i14bgtn5okxt-3>li:before{content:"\0025cf "}.lst-kix_i14bgtn5okxt-6>li:before{content:"\0025cf "}ul.lst-kix_i14bgtn5okxt-0{list-style-type:none}ul.lst-kix_i14bgtn5okxt-5{list-style-type:none}ul.lst-kix_i14bgtn5okxt-6{list-style-type:none}.lst-kix_i14bgtn5okxt-4>li:before{content:"\0025cb "}ul.lst-kix_i14bgtn5okxt-3{list-style-type:none}ul.lst-kix_i14bgtn5okxt-4{list-style-type:none}ul.lst-kix_i14bgtn5okxt-7{list-style-type:none}ul.lst-kix_i14bgtn5okxt-8{list-style-type:none}.lst-kix_i14bgtn5okxt-8>li:before{content:"\0025a0 "}.lst-kix_i14bgtn5okxt-7>li:before{content:"\0025cb "}.lst-kix_i14bgtn5okxt-1>li:before{content:"\0025cb "}.lst-kix_i14bgtn5okxt-5>li:before{content:"\0025a0 "}.lst-kix_i14bgtn5okxt-2>li:before{content:"\0025a0 "}.lst-kix_i14bgtn5okxt-0>li:before{content:"\0025cf "}ol{margin:0;padding:0}.c0{padding-left:0pt;widows:2;orphans:2;direction:ltr;margin-left:36pt}.c2{padding-left:0pt;widows:2;orphans:2;direction:ltr;margin-left:72pt}.c1{padding-left:0pt;widows:2;orphans:2;direction:ltr;margin-left:108pt}.c4{max-width:468pt;background-color:#ffffff;padding:72pt 72pt 72pt 72pt}.c3{margin:0;padding:0}.title{widows:2;padding-top:0pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:21pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}.subtitle{widows:2;padding-top:0pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-style:italic;font-size:13pt;font-family:"Trebuchet MS";padding-bottom:10pt;page-break-after:avoid}li{color:#000000;font-size:11pt;font-family:"Arial"}p{color:#000000;font-size:11pt;margin:0;font-family:"Arial"}h1{widows:2;padding-top:10pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:16pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h2{widows:2;padding-top:10pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:13pt;font-family:"Trebuchet MS";font-weight:bold;padding-bottom:0pt;page-break-after:avoid}h3{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:12pt;font-family:"Trebuchet MS";font-weight:bold;padding-bottom:0pt;page-break-after:avoid}h4{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:11pt;text-decoration:underline;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h5{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:11pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h6{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-style:italic;font-size:11pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}</style></head><body class="c4">
  • We tried the snakeskin dialysis tubing again:
  • We cut a 12 mL length of tubing
  • twisted one end of the tubing, and tied it off using twine
  • folded that end over once
  • used a binder clip to clip the end
  • loaded ~12mL of silk into the tubing
  • then used the same method to seal off the other end
  • then put the entire thing in nanopure water to dialyze
  • dialysis start: 4:30 pm, May 5
  • We also dissolved 2.41 more grams of silk
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