UCLAiGEM:Notebook/MaterialsProcessing/2015/04/23

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<html><head><title>23 April </title><meta content="text/html; charset=UTF-8" http-equiv="content-type"><style type="text/css">.lst-kix_bvhhqr48khi6-3>li:before{content:"\0025cf "}.lst-kix_9dpvo8diy0sv-6>li:before{content:"\0025cf "}ul.lst-kix_9dpvo8diy0sv-8{list-style-type:none}.lst-kix_9dpvo8diy0sv-3>li:before{content:"\0025cf "}.lst-kix_bvhhqr48khi6-7>li:before{content:"\0025cb "}.lst-kix_9dpvo8diy0sv-2>li:before{content:"\0025a0 "}.lst-kix_bvhhqr48khi6-8>li:before{content:"\0025a0 "}.lst-kix_9dpvo8diy0sv-8>li:before{content:"\0025a0 "}.lst-kix_bvhhqr48khi6-5>li:before{content:"\0025a0 "}.lst-kix_bvhhqr48khi6-0>li:before{content:"\0025cf "}.lst-kix_bvhhqr48khi6-4>li:before{content:"\0025cb "}.lst-kix_9dpvo8diy0sv-7>li:before{content:"\0025cb "}.lst-kix_9dpvo8diy0sv-4>li:before{content:"\0025cb "}ul.lst-kix_bvhhqr48khi6-7{list-style-type:none}ul.lst-kix_bvhhqr48khi6-6{list-style-type:none}.lst-kix_9dpvo8diy0sv-1>li:before{content:"\0025cb 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"}ul.lst-kix_9dpvo8diy0sv-1{list-style-type:none}ol{margin:0;padding:0}.c4{border-bottom-width:1pt;border-top-style:solid;width:77.2pt;border-right-style:solid;padding:5pt 5pt 5pt 5pt;border-bottom-color:#000000;border-top-width:1pt;border-bottom-style:solid;vertical-align:top;border-top-color:#000000;border-left-color:#000000;border-right-color:#000000;border-left-style:solid;border-right-width:1pt;border-left-width:1pt}.c3{border-bottom-width:1pt;border-top-style:solid;width:238.5pt;border-right-style:solid;padding:5pt 5pt 5pt 5pt;border-bottom-color:#000000;border-top-width:1pt;border-bottom-style:solid;vertical-align:top;border-top-color:#000000;border-left-color:#000000;border-right-color:#000000;border-left-style:solid;border-right-width:1pt;border-left-width:1pt}.c7{border-bottom-width:1pt;border-top-style:solid;width:116.2pt;border-right-style:solid;padding:5pt 5pt 5pt 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class="c5"><span>This time: we made 3 more gels (all 1.5 mL)</span></li><li class="c5"><span>We casted the gels at 5:40 pm, 23 April.</span></li><li class="c5 c9"><span></span></li></ul><a href="#" name="ddfb7a0015a595361b6d51d8c896b3921d9b3c57"></a><a href="#" name="0"></a><table cellpadding="0" cellspacing="0" class="c11"><tbody><tr class="c10"><td class="c4" colspan="1" rowspan="1"><p class="c0"><span class="c1">silk % (w/v)</span></p></td><td class="c7" colspan="1" rowspan="1"><p class="c0"><span class="c1">treatment</span></p></td><td class="c3" colspan="1" rowspan="1"><p class="c0"><span class="c1">Comments</span></p></td></tr><tr class="c10"><td class="c4" colspan="1" rowspan="1"><p class="c0"><span class="c1">6.4</span></p></td><td class="c7" colspan="1" rowspan="1"><p class="c0"><span class="c1">nothing</span></p></td><td class="c3" colspan="1" rowspan="1"><p class="c0 c9"><span class="c1"></span></p></td></tr><tr class="c10"><td class="c4" colspan="1" rowspan="1"><p class="c0"><span class="c1">6.4</span></p></td><td class="c7" colspan="1" rowspan="1"><p class="c0"><span class="c1">vortexed, max speed, 7 minutes</span></p></td><td class="c3" colspan="1" rowspan="1"><p class="c0"><span class="c1">After vortexing, the solution had particulates floating in it. This may be because the concentration was too high -- the Kaplan protocol recommended going up to only 5% w/v.</span></p></td></tr><tr class="c10"><td class="c4" colspan="1" rowspan="1"><p class="c0"><span class="c1">0.8</span></p></td><td class="c7" colspan="1" rowspan="1"><p class="c0"><span class="c1">vortexed, max speed, 7 minutes</span></p></td><td class="c3" colspan="1" rowspan="1"><p class="c0"><span class="c1">Yeah, this is hardly any silk at all. We made it to use up the remaining solution that we had left.</span></p></td></tr></tbody></table><ul class="c8 lst-kix_9dpvo8diy0sv-0"><li class="c5"><span style="overflow: hidden; display: inline-block; margin: 0.00px 0.00px; border: 0.00px solid #000000; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px); width: 451.33px; height: 338.50px;"><img alt="IMG_0007.JPG" src="http://openwetware.org/images/a/ad/Silk_hydrogel_UCLA_23_April_15.JPG" style="width: 451.33px; height: 338.50px; margin-left: 0.00px; margin-top: 0.00px; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px);" title=""></span></li><li class="c5"><span>We also degummed 2.5 more grams of silk</span></li></ul><p class="c2"><span></span></p></body></html>