Talk:CH391L/S2013 Tanya Raymond Feb 6 2013

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Taylor: This may be a silly/stupid question but why is the restarted fork more error prone initially but becomes less so as it continues replication? (Figure 3a-c)

  • Tanya Raymond This is a great question. What makes a replication fork error prone after it has restarted has to do with the homologous recombination machinery's search for a homologous region of sequence to use to repair the fork. This result shows that the HR machinery is more likely to be able to efficiently restart without error when the palindrome sequence is farther away from the restarting fork. This paper showed that the palindrome sequence is the error-inducing region of sequence since there is much homology that can be used erroneously by the HR machinery. My thinking is that with more distance between the palindrome and the restarting fork, there is a longer length of DNA for the HR machinery to use to allow for restart on the correct strand.