Talk:20.109(S11):Prepare DNA for cloning (Day3)

T/R

Record of purification gel

T/R Red, Orange, Yellow Groups

T/R Green, Blue, Pink, Purple Groups

Control digest gel

If you digested for longer than 1 hr or otherwise changed the protocol, please add that to the "Notes" section in the table below.

Control digest gel; samples described in table at right.

Lane #	Group	Sample	Notes
1	Red	XmaI
2	Orange	BamHI
3	Orange	BamHI
4	Yellow	XmaI
5	N/A	Undigested	2.5 μL DNA
6	N/A	10 μL Ladder	1 Kbp size from NEB
7	Green	BamHI
8	Blue	XmaI
9	Pink	BamHI
10	Purple	XmaI

W/F

Record of purification gel

W/F Red, Orange, Yellow Groups

W/F Green, Blue, Pink, Purple Groups

Control digest gel

If you digested for longer than 1 hr or otherwise changed the protocol, please add that to the "Notes" section in the table below.

Control digest gel; samples described in table at right.

Lane #	Group	Sample	Notes
1	N/A	10 μL Ladder	1 Kbp size from NEB
2	Red	XmaI
3	Orange	BamHI
4	Yellow	XmaI
5	N/A	Undigested	2.5 μL DNA
6	Green	BamHI
7	Blue	XmaI
8	Pink	BamHI
9	Purple	XmaI
10	N/A	10 μL Ladder	1 Kbp size from NEB