Talk:20.109(S08):Testing cell viability (Day3)

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Please write your team colour next to the spot that you would like.

Feel free to arrive earlier than your scheduled time!

T/R schedule

Microscope time Arrival time (at latest!) Team colour
2:30-2:55 1:05 Yellow
2:55-3:20 1:25 Pink
3:20-3:45 1:50 Red
3:45-4:10 2:15 Purple
4:10-4:35 2:40 Green
4:35-5:00 3:05 Blue

W/F schedule

Microscope time Arrival time (at latest!) Team colour
2:30-2:55 1:05 Purple
2:55-3:20 1:25 Red
3:20-3:45 1:50 Blue
3:45-4:10 2:15 Green
4:10-4:35 2:40 Pink
4:35-5:00 3:05 Yellow

T/R pictures

Please post one picture for each of your two alginate samples (you can post just the green channel).

Caption for Agi's example pictures, 40X, used 1% alginate
Figure: Yellow Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no collagen II.


Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with no toxin added.
Figure: Pink Group. 10X FITC filter. 3D 1% alginate chondrocyte cells with toxin added.
Figure: Pink Group. 10X FITC filter. 2D chondrocyte cells with rich media.
Figure: Red Group. 10X FITC filter. 2D chondrocyte cells with media w/o ascorbate.
Figure: Red Group. 10X FITC filter. 3D 2% Sigma Aldrich alginate in rich media.
Figure: Purple Group. 10X FITC filter. 2D sample.
Figure: Green Group. 10X FITC filter. 2D sample.
Figure: Green Group. 10X FITC filter. 3D sample. 5million in 1%alginate
Figure: Green Group. 10X FITC filter. 2D sample. 20million in 1%alginate
Figure: Blue Group. 10X FITC filter. 2D sample. 1million cells


W/F pictures

Please post one picture for each of your two alginate samples (you can post just the green channel).

Caption for Agi's example pictures, 40X, used 1% alginate
Purple Group. Left Figure: 10X FITC Filter, 2D Sample. Right Figure: 10X EthD-1 Filter, 3D Sample in 1% alginate
Red Group. Left figure: 10X FITC filter, 3D with mechanical stress, 1% alginate. Right figure: 10X FITC Filter, 2D 1% alginate
Blue Group. 10X FITC filter, 3D normal calcium concentration, 2% alginate
Blue Group. 10X EthD-1 Filter, 3D normal calcium concentration, 2% alginate
Green Group. 10X FITC filter, 2D normal culture.
Green Group. 10X FITC filter, 3D 2% Sigma brand low viscosity alginate culture.
Pink Group. 10X FITC filter, control culture in 1% alginate.
Pink Group. 10X FITC filter, weighted culture in 1% alginate. Weights were used to test response of chondrocyes to mechanical stress.
Yellow Group. 2D sample 10X
Yellow Group. 2D sample 40X
Yellow Group. 3D sample 10X