Talk:20.109(S08):Start-up protein engineering (Day1)

TR lab

Team colour	Mutant 1	Primer 1 (forward)	Mutant 2	Primer 2 (forward)
Red	D133R	GGGAAGCAGATATCGATGGTCGTGGCCAAGTAAACTATGAAG	D133W	GGGAAGCAGATATCGATGGTTGGGGCCAAGTAAACTATGAAGAG
Purple	Y64P	GCTGATGGCAATGGAACGATTCCCTTTCCTGAATTTCTTACTATG	Y64K	GCTGATGGCAATGGAACGATTAAGTTTCCTGAATTTCTTACTATG
Blue	G98D	GACAAGGATGGGAACGACTACATCAGCGCTGCTCAGTTACGTC	G98P	GACAAGGATGGGAACCCCTACATCAGCGCTGCTCAGTTACGTC
Pink	G96D	CGTGTTTTTGACAAGGATGACAACGGCTACATCAGCGCTGC	G96K	GTTTTTGACAAGGATAAGAACGGCTACATCAGCGCTGCTCAG
Yellow	T28D	CGACAAGGATGGGGACGGCACCATCGACACAAAGGAAC	T28R	GATGGGGACGGCACCATCAGGACAAAGGAACTTGGCACCG
Green	Y64D	GGCAATGGAACGATTGACTTTCCTGAATTTCTGACTATGATGGC	Y64H	GGCAATGGAACGATTCACTTTCCTGAATTTCTCACTATGATGGC

WF lab

Team colour	Mutant 1	Primer 1 (forward)	Mutant 2	Primer 2 (forward
PINK	D20->P	CTTCTCATTATTCCCCAAGGATGGGGACGGCACCATCACC	D20->R	CTTCTCATTATTCCGCAAGGATGGGGACGGCACCATCACC
PURPLE	S101->L	GATGGGAACGGCTACATCCTCGCTGCTCAG	S101->T	GATGGGAACGGCTACATCACCGCTGCTCAG
BLUE	N60->E	GATGCTGATGGCGAAGGAACGATTTAC	N60->K	GATGCTGATGGCAAGGGAACGATTTAC
RED	G98->P	GTTTTTGACAAGGATGGGAACCCCTACATCAGCGCTGCTCAG	G98->W	GTTTTTGACAAGGATGGGAACTGGTACATCAGCGCTGCTCAG
Yellow	D56H	5' GAT ATG ATC AAT GAA GTC CAT GCT GAT GGC AAT GGA ACG 3'	D56I	5' GAT ATG ATC AAT GAA GTC ATT GCT GAT GGC AAT GGA ACG 3'
GREEN	K94->H	gcattccgtgtttttgacCACgatgggaacggctacatcagcgc	K94->A	gcattccgtgtttttgacGCGgatgggaacggctacatcagcgc

All class mutations

1                                                                                                99
TDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIYFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGY
                   P       D                           H   E   P                             H D D
                   R       R                           I   K   D                             A K P
                                                               K                                 P
                                                               H                                 W

 
100                                           148
ISAAQLRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
 L                               R              
 T                               W