Talk:20.109(F11): Mod 1 Day 3 Agarose gel electrophoresis
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
You can use these in your lab report if you so choose.
![](https://s3-us-west-2.amazonaws.com/oww-files-public/1/1d/FragsMod1.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-public/2/22/Excisedfrag.jpg)
T/R lab
Red | Orange | Yellow | Green |
Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
Tuesday/Thursday Gel 1:
Lane 1: Red Fragment
Lane 2: Red Backbone
Lane 3:Orange Fragment
Lane 4:Orange Backbone
Lane 5: Marker
Lane 6: Yellow Fragment
Lane 7:Yellow Backbone
Lane 8:Green Fragment
Lane 9:Green Backbone
Lane 10:Blank
Tuesday/Thursday Gel 2:
Lane 1: Blue Fragment
Lane 2: Blue Backbone
Lane 3:Pink Fragment
Lane 4:Pink Backbone
Lane 5: Marker
Lane 6: Purple Fragment
Lane 7:Purple Backbone
Lane 8-10:Blank
W/F Lab
Red | Orange | Yellow | Green |
Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
Note: All markers contain 15uL instead of 10.
Wednesday/Friday Gel 1:
Lane 1: Red Fragment
Lane 2: Red Backbone
Lane 3: Marker
Lane 4:Orange Fragment
Lane 5:Orange Backbone
Lane 6: Marker
Lane 7: Yellow Fragment
Lane 8:Yellow Backbone
Wednesday/Friday Gel 2:
Lane 1:Green Fragment
Lane 2:Blue Backbone
Lane 3:Marker
Lane 4: Blue Fragment
Lane 5: Blue Backbone
Lan3 6:Marker
Lane 7:Pink Fragment
Lane 8:Pink Backbone
Here are the pre-purification gels for reference
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/c/cc/F11_M1D3_WF-Blu.jpg/250px-F11_M1D3_WF-Blu.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/9/9e/F11_M1D3_WF-Grn.jpg/250px-F11_M1D3_WF-Grn.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/6/6e/F11_M1D3_WF-Org.jpg/250px-F11_M1D3_WF-Org.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/f/fd/F11_M1D3_WF-Pnk.jpg/250px-F11_M1D3_WF-Pnk.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/d/db/F11_M1D3_WF-Red.jpg/250px-F11_M1D3_WF-Red.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/8/87/F11_M1D3_WF-Ylw.jpg/250px-F11_M1D3_WF-Ylw.jpg)