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|
Antibiotic Concentrations and Usage
| Name |
Stock Concentration (mg/ml) |
Final Concentration (μg/ml) |
Usage in 10mL (μL) |
Dissolve in
|
| Ampicillin |
100 |
100 |
10 |
sdH20
|
| Apramycin |
100 |
50 |
5 |
sdH20
|
| Chloramphenicol |
25 |
25 |
10 |
50%-100% EtOH
|
| Hygromycin |
50 |
25 |
5 |
sdH20
|
| Kanamycin |
100 |
50 |
5 |
sdH20
|
| Nalidixic Acid |
25 |
25 |
10 |
0.2M NaOH
|
| Thiostrepton(e) |
50 |
50 |
10 |
100% DMSO or 1:1 DMSO:EtOH
|
| Viomycin |
30 |
30 |
10 |
sdH20
|
E.coli Strain & Plasmid Resistance Markers
| E.coli Strain |
Plasmid / Cosmid |
E.coli Resistance Marker |
Plasmid Resistance Marker |
Temperature Sensitive Replicon
(ts)
|
| BW25113 |
pIJ790 (λRED) |
None |
cat |
Replication @ 30°C,
Loss of λRED @ 37°C
|
| DH5α |
None |
N/A |
|
|
| DH5α |
BT340 (FLP Recombinase) |
None |
cat / blacarb |
Initial Replication @ 30°C,
Induction of FLP Recombinase & loss of plasmid @ 42°C,
Post recombination growth @37°C
|
ET12567
[dam-13::Tn9] |
pUZ8002 |
cat |
neo |
|
|
SuperCos 1 |
|
neo / blacarb |
|
|
pIJ773 |
|
acc(3)IV |
|
Antibiotic Genes & Information
| Antibiotic Name |
Gene Name |
Type |
Common Form
|
| Ampicillin |
bla |
Bactericidal |
Sodium
|
| Apramycin |
aac(3)IV |
Aminoglycoside |
Sulphate
|
| Carbenicillin |
blacarb |
Bactericidal |
DiSodium
|
| Chloramphenicol |
cat |
Bacteriostatic |
|
| Erythromycin |
ermE |
Macrolides |
|
| Hygromycin B |
hph |
Aminoglycoside |
|
| Kanamycin / Neomycin |
neo |
Aminoglycoside |
MonoSulphate
|
| Nalidixic Acid |
Synthetic |
Bacteriostatic |
|
| Spectinomycin / Streptomycin |
aadA |
Aminocyclitol |
DiHydrochloride Pentahydrate
|
| Tetracyclin |
Tet |
Aminoglycoside |
Hydrochloride
|
| Thiostrepton |
tsr |
Aminoglycoside |
|
| Viomycin |
vph |
Aminoglycoside? |
Sulphate
|
| Vancomycin B |
vanB |
Glycopeptide |
Hydrochloride
|
|
