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McClean: Designing "Yeast Toolkit" compatible primers: Difference between revisions
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McClean: Designing "Yeast Toolkit" compatible primers (view source)
Revision as of 09:22, 15 September 2016
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* 3'- CGTAGCAGAGTAGCCAGAGTNNNN- gene of interest - NNNNA'''CTCTGG'''A'''CTCTGC'''CGTA -5' | * 3'- CGTAGCAGAGTAGCCAGAGTNNNN- gene of interest - NNNNA'''CTCTGG'''A'''CTCTGC'''CGTA -5' | ||
* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . <--- Reverse Primer | * . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . <--- Reverse Primer | ||
==Notes== | ==Notes== | ||
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==References== | ==References== | ||
''Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synthetic Biology at <http://pubs.acs.org/doi/pdf/10.1021/sb500366v> (2015)'' | |||
==Contact== | ==Contact== | ||
<!--Change the information below to your info if you add a new protocol--> | <!--Change the information below to your info if you add a new protocol--> | ||
*'''[[User:Megan N McClean|Megan N McClean]] 14:01, 20 July 2011 (EDT)''' | *'''[[User:Megan N McClean|Megan N McClean]] 14:01, 20 July 2011 (EDT)''' | ||
*'''[[User:Cameron J. Stewart|Cameron J. Stewart]] 12:22, 15 September 2016 (EDT)''': | |||
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | ||
