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McClean: Designing "Yeast Toolkit" compatible primers: Difference between revisions
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McClean: Designing "Yeast Toolkit" compatible primers (view source)
Revision as of 09:06, 15 September 2016
, 15 September 2016→Protocol
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* 5'- GCAT'''CGTCTC'''ATC'''GGTCTC'''ANNNN- gene of interest - NNNNTGAGACCTGAGACGGCAT -3' | * 5'- GCAT'''CGTCTC'''ATC'''GGTCTC'''ANNNN- gene of interest - NNNNTGAGACCTGAGACGGCAT -3' | ||
* 3'- CGTAGCAGAGTAGCCAGAGTNNNN- gene of interest - NNNNA'''CTCTGG'''A'''CTCTGC'''CGTA -5' | * 3'- CGTAGCAGAGTAGCCAGAGTNNNN- gene of interest - NNNNA'''CTCTGG'''A'''CTCTGC'''CGTA -5' | ||
* . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . <--- Reverse Primer | * . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . <--- Reverse Primer | ||
# Step | # Step | ||
# Step 3 | # Step 3 | ||
==Notes== | ==Notes== | ||
