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E. coli genotypes: Difference between revisions
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Added JTK165
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===DH1=== | ===DH1=== | ||
endA1 recA1 gyrA96 thi-1 glnV44 | F<sup>–</sup> λ<sup>–</sup> ''endA1'' ''recA1'' ''relA1'' ''gyrA96'' ''thi-1'' ''glnV44'' ''hsdR17''(''r<sub>K</sub>''<sup>–</sup>''m<sub>K</sub>''<sup>–</sup>) | ||
* | * Parent of DH5α | ||
* | * A Hoffman-Berling 1100 strain derivative (Meselson68) | ||
* | * More efficient at transforming large (40-60Kb) plasmids | ||
* | * Thiamine auxotroph | ||
* Lactose nonutilizing | |||
* Nalidixic acid resistant | |||
* Recombination-deficient | |||
* Relaxed phenotype | |||
* Endonuclease A deficient | |||
*Reference: Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368. | *Reference: Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368. | ||
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*This strain is no longer available from the CGSC | *This strain is no longer available from the CGSC | ||
* This strain may also be F-. JM2.300 is described as F-, lacI22, λ-, e14-, rpsL135(StrR), thi-1 in K. Brenner, D. Karig, R. Weiss, F. Arnold, “Engineered biodirectional communication mediates a consensus in a microbial biofilm consortium,” in Proc. Natl. Acad. Sci. USA, 2007 Oct 30;104(44):17300-4. | * This strain may also be F-. JM2.300 is described as F-, lacI22, λ-, e14-, rpsL135(StrR), thi-1 in K. Brenner, D. Karig, R. Weiss, F. Arnold, “Engineered biodirectional communication mediates a consensus in a microbial biofilm consortium,” in Proc. Natl. Acad. Sci. USA, 2007 Oct 30;104(44):17300-4. | ||
===JTK165=== | |||
str. MC1061 Δ''upp'':(''pir'' ''dhfr''(Tmp<sup>R</sup>)) Δ(''rfbB''–''wbbL''')::''repA''<br><br> | |||
More detailed:<br> | |||
str. MC1061<small>[K-12 F<sup>–</sup> λ<sup>–</sup> Δ''(ara-leu)7697'' [''araD139'']B/r Δ''(codB-lacI)3'' ''galK16'' ''galE15'' e14<sup>–</sup> ''mcrA0'' ''relA1'' ''rpsL150''(Str<sup>R</sup>) ''spoT1'' ''mcrB1'' ''hsdR2''(''r''<sup>–</sup>''m''<sup>+</sup>)]</small> Δ''upp''::(P<sub>con</sub>(''BBa_J23100'')-rbs(?)-''pir''-''BBa_B0015''(''rrfBt''(''rrnBt'')+''T7t'') - ''FRT'' - P<sub>con</sub>(''BBa_J23119'')-''4krbs''-''dhfr'' (Tmp<sup>R</sup>)-''tR2'' - ''FRT'') Δ(''rfbB''–''wbbL''')::(P<sub>con</sub>(''BBa_J23119'')-rbs(?)-''repA''-''BBa_B0015''(''rrfBt''(''rrnBt'')+''T7t'') - ''FRT'') | |||
*Propagates R6K- and ColE2-origin plasmids by expressing ''pir'' and ''repA'', respectively. | |||
*Relaxed phenotype | |||
*''lacI''<sup>–</sup> | |||
*Improved cloning 5-mC DNA | |||
*Str<sup>R</sup> Spc<sup>R</sup> Tmp<sup>R</sup>(FRT-flanked) | |||
*Leucine auxotroph | |||
*Galactose, lactose, arabinose nonutilizing | |||
*Generic DIAL strain (Kittleson & Anderson 2011) <biblio>pmid=21787416</biblio> | |||
===K12 3000=== | ===K12 3000=== | ||
''hfrH'' ''relA1'' ''spoT1'' ''thi-1'' | |||
* This strain is designed for propagation of F+ specific phages | * This strain is designed for propagation of F+ specific phages | ||
*See [http://bccm.belspo.be/catalogues/files/lmbp-hosts/k12%203000.pdf LMBP 1393] | *See [http://bccm.belspo.be/catalogues/files/lmbp-hosts/k12%203000.pdf LMBP 1393] | ||
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===MC1061=== | ===MC1061=== | ||
F<sup>–</sup> λ<sup>–</sup> Δ''(ara-leu)7697'' [''araD139'']B/r Δ''(codB-lacI)3'' ''galK16'' ''galE15'' e14<sup>–</sup> ''mcrA0'' ''relA1'' ''rpsL150''(Str<sup>R</sup>) ''spoT1'' ''mcrB1'' ''hsdR2''(''r''<sup>–</sup>''m''<sup>+</sup>) | str. K-12 F<sup>–</sup> λ<sup>–</sup> Δ''(ara-leu)7697'' [''araD139'']B/r Δ''(codB-lacI)3'' ''galK16'' ''galE15'' e14<sup>–</sup> ''mcrA0'' ''relA1'' ''rpsL150''(Str<sup>R</sup>) ''spoT1'' ''mcrB1'' ''hsdR2''(''r''<sup>–</sup>''m''<sup>+</sup>) | ||
*Streptomycin resistant | *Streptomycin resistant | ||
*Relaxed phenotype | *Relaxed phenotype | ||
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===MC4100=== | ===MC4100=== | ||
F<sup>-</sup> [araD139]<sub>B/r</sub> Δ(argF-lac)169* &lambda<sup>-</sup> e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(:: | F<sup>-</sup> [araD139]<sub>B/r</sub> Δ(argF-lac)169* &lambda<sup>-</sup> e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS''1'') ''deoC1'' | ||
*The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain construction. | *The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain construction. | ||
