E. coli genotypes: Difference between revisions

E. coli genotypes (view source)

66 bytes added , 4 April 2016

m

26

edits

@@ Line 186: / Line 186: @@
 ===NEB Turbo (NEB)===
-F′ ''proA''<sup>+</sup>''B''<sup>+</sup> ''lacI<sup>q</sup>'' ∆''lacZM15''/''fhuA2'' ∆(''lac-proAB'') ''glnV'' ''galE15'' ''galK16'' ''R''(''zgb-210''::Tn''10'')Tet<sup>S</sup>  ''endA1'' ''thi-1'' ∆''(hsdS-mcrB)5''
+''glnV44'' ''thi-1'' Δ''(lac-proAB)'' ''galE15'' ''galK16'' ''R''(''zgb-210''::Tn''10'')Tet<sup>S</sup>  ''endA1'' Δ''(mcrB-hsdSM)5'', (''r<sub>K</sub>''<sup>–</sup>''m<sub>K</sub>''<sup>–</sup>) F′ [''traD36'' ''proAB''<sup>+</sup> ''lacI''<sup>q</sup> ''lacZΔM15''/''fhuA2'']
-* TG1 derivative, based on very similar genotype and fast growth phenotype
+* TG1 derivative, based on very similar genotype and fast growth.
 ** T<sub>1</sub> phage resistant
 ** Thiamine auxotroph
 ** Lactose nonutilizing
-* Rapid growth: visible colonies on agar, ~6.5 hours; shaking liquid culture OD 600 = 2.0, ~4 hours
+** Rapid growth: visible colonies on agar, ~6.5 hours; shaking liquid culture OD 600 = 2.0 in ~4 hours
 * Endonuclease A-deficient
 * Galactose nonutilizing
-* Expresses the Lac repressor
+* Constitutive ''lac'' repressor LacI
 * References:
 ** New England Biolabs, [https://www.neb.com/products/C2984-NEB-Turbo-Competent-E-coli-High-Efficiency product catalogue number C2984H]