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Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions
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Knight:Purification of His-tagged proteins/Denaturing (view source)
Revision as of 18:45, 12 December 2006
, 12 December 2006→Notes
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*These columns can tolerate up to 20mM β-mercaptoethanol or 1mM dithiothreitol. | *These columns can tolerate up to 20mM β-mercaptoethanol or 1mM dithiothreitol. | ||
*Even when doing denaturing purifications, add 10mM imidazole to solutions to help with washing out non His tagged proteins. | *Even when doing denaturing purifications, add 10mM imidazole to solutions to help with washing out non His tagged proteins. | ||
==Troubleshooting and optimization== | |||
*[[Knight:Deionization|Deionize]] urea solutions prior to use. | |||
*Degas all buffers. | |||
==Safety== | ==Safety== | ||
