Talk:Knight:Purification of His-tagged proteins/Denaturing
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Sample results
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/d/d7/KnightNuPAGEBisTrisgelmicrowavestain.jpg/400px-KnightNuPAGEBisTrisgelmicrowavestain.jpg)
Lane order:
- SeeBlue Plus2 Pre-Stained Standard
- Cleared lysate
- Flow through (after column binding)
- Flow through (after column binding, second time)
- Wash 1
- Wash 2
- Eluted protein
- Cleared lysate from cells not expressing a His-tagged protein
- Flow through (after column binding, second time) from cells not expressing a His-tagged protein
- Wash 2 from cells not expressing a His-tagged protein