Talk:Knight:Purification of His-tagged proteins/Denaturing

Sample results

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Denaturing PAGE of ~13kD His tagged protein purified via this method.

Lane order:

1. SeeBlue Plus2 Pre-Stained Standard
2. Cleared lysate
3. Flow through (after column binding)
4. Flow through (after column binding, second time)
5. Wash 1
6. Wash 2
7. Eluted protein
8. Cleared lysate from cells not expressing a His-tagged protein
9. Flow through (after column binding, second time) from cells not expressing a His-tagged protein
10. Wash 2 from cells not expressing a His-tagged protein