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BME100 f2014:Group31 L4: Difference between revisions
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INITIAL STEP: 95°C for 2 minutes | INITIAL STEP: 95°C for 2 minutes | ||
This initial step is done to denature the DNA, splitting the DNA into two separate strands, thus preparing it for replication. | |||
NUMBER OF CYCLES: 35 | NUMBER OF CYCLES: 35 | ||
Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds | Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds | ||
[[Image:CHELSEA.png]] | |||
This image depicts the effect and purpose reducing the temperature to 95 degrees C, which is to denature the DNA so that it splits into two separate strands. After the solution is cooled to 57 degrees C, the temperature is right for the primers to anneal to each strand of DNA. | |||
FINAL STEP: 72°C for 2 minutes | |||
[[Image:Pcr3.JPG]] | [[Image:Pcr3.JPG]] | ||
This image depicts the overall purpose of heating the solution to 72 degrees C, which is that it is the ideal temperature to activate the Taq Polymerase, which then extends and replicates the DNA. | |||
FINAL HOLD: 4°C | FINAL HOLD: 4°C | ||
