BME100 f2014:Group31 L4: Difference between revisions

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	N/A. ''DNA Engine® Multi-Bay Thermal Cyclers — Alpha™ Unit Reaction Modules''. Digital Image. ''Bio-Rad''. Bio-Rad Laboratories Inc., N/A. Web. 10/27/14.		N/A. ''DNA Engine® Multi-Bay Thermal Cyclers — Alpha™ Unit Reaction Modules''. Digital Image. ''Bio-Rad''. Bio-Rad Laboratories Inc., N/A. Web. 10/27/14.

			The thermal cycling program multiplies a certain DNA sequence by an enormous amount. This occurs using a combination of primers, Taq Polymerase, and rapid heating and cooling. The program has two main settings: Primer Annealing and Primer Extension. The Annealing stage changes depending on sequence content, length of sequence, and primer concentration. The other setting, Extension, will change based on length of sequence and temperature. The amount of cycles depends on the amount of DNA to begin with.