BME100 f2014:Group31 L4

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Charles Bolton Name: Andy Chang Name: Michael Chatarachanwong Name: Afshin Isadvesta Name: Andrew Liu Name: Jimmy Xu LAB 4 WRITE-UP Protocol Materials Lab coat and disposable gloves PCR reaction mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's (http://www.promega.com/resources/protocols/product-information-sheets/g/gotaq-colorless-master-mix-m714-protocol DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer A strip of empty PCR tubes Disposable pipette tips: only use each only once. Never re-use disposable pipette tips or samples will be cross-contaminated Cup for discarded tips Micropipettor OpenPCR machine: shared by two groups PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G# + Positive control none G# - Negative control none G# 1-1 Patient 1, replicate 1 G# 1-2 Patient 1, replicate 2 G# 1-3 Patient 1, replicate 3 G# 2-1 Patient 2, replicate 1 G# 2-2 Patient 2, replicate 2 G# 2-3 Patient 2, replicate 3 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... OpenPCR program Research and Development PCR - The Underlying Technology