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BME100 f2014:Group31 L4: Difference between revisions
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'''Materials''' | '''Materials''' | ||
<!-- Record all of the materials you will need for PCR --> | <!-- Record all of the materials you will need for PCR --> | ||
* | * Lab coat and disposable gloves | ||
* | * PCR reaction mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's (http://www.promega.com/resources/protocols/product-information-sheets/g/gotaq-colorless-master-mix-m714-protocol | ||
* | * DNA/ primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer | ||
* A strip of empty PCR tubes | |||
* Disposable pipette tips: only use each only once. Never re-use disposable pipette tips or samples will be cross-contaminated | |||
* Cup for discarded tips | |||
* Micropipettor | |||
* OpenPCR machine: shared by two groups | |||
