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Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions
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Knight:Purification of His-tagged proteins/Denaturing (view source)
Revision as of 15:13, 15 October 2006
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*Contaminating proteins tend to be less of an issue in bacteria because there are few proteins with neighboring histidines that tend to bind to the column. | *Contaminating proteins tend to be less of an issue in bacteria because there are few proteins with neighboring histidines that tend to bind to the column. | ||
*20 year old spin columns don't work. :) | *20 year old spin columns don't work. :) | ||
*These columns can tolerate up to 20mM β-mercaptoethanol or 1mM dithiothreitol. | |||
==Safety== | ==Safety== | ||
