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Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions
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Knight:Purification of His-tagged proteins/Denaturing (view source)
Revision as of 08:46, 5 October 2006
, 5 October 2006→Procedure
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#*''I stored the pellet at -80°C.'' | #*''I stored the pellet at -80°C.'' | ||
#Thaw for 30 mins. | #Thaw for 30 mins. | ||
#*'' | #*''Can thaw at room temperature since the next step happens at room temperature. However, proteases will be less active if the pellet is thawed on ice.'' | ||
#*''The Qiagen protocol calls for 15 mins, but it was still frozen after 15 mins so I let it thaw for 30 mins.'' | #*''The Qiagen protocol calls for 15 mins, but it was still frozen after 15 mins so I let it thaw for 30 mins.'' | ||
#Transferred to 2mL eppendorf tube. | #Transferred to 2mL eppendorf tube. | ||
