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Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions
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Knight:Purification of His-tagged proteins/Denaturing (view source)
Revision as of 08:34, 5 October 2006
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===Notes=== | ===Notes=== | ||
*Solid urea to make up an 8M solution takes up a lot of volume so be conservative on how much H <sub>2</sub>O you start with (maybe 50% of final volume) | *Solid urea to make up an 8M solution takes up a lot of volume so be conservative on how much H <sub>2</sub>O you start with (maybe 50% of final volume) | ||
*Kathleen suggested supplementing the lysis buffer with 10mM imidazole to prevent nonspecific protein binding to the column. | *Kathleen suggested supplementing the lysis buffer with 10mM imidazole to prevent nonspecific protein binding to the column. | ||
