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Lidstrom:Buffers: Difference between revisions
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→Notes specific to buffers
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== Notes specific to buffers == | == Notes specific to buffers == | ||
=== phosphate buffer === | === phosphate buffer === | ||
* Phosphates, for example, form insoluble salts with bivalent metals and precipitate. Phosphate buffered salt solution (PBS) is never | * Phosphates, for example, form insoluble salts with bivalent metals and precipitate. Phosphate buffered salt solution (PBS) is never autoclaved with Ca2+ or Mg2+ for this reason. Good buffers, such as PIPES, TES, HEPES and CAPS have very low metal-binding constants and are therefore particularly suited to investigate metal-dependent enzymes (Good & Izawa 1972, Blanchard 1984). [http://www.applichem.com/fileadmin/Broschueren/BioBuffer.pdf Applichem] | ||
autoclaved with Ca2+ or Mg2+ for this reason. Good buffers, such as PIPES, TES, HEPES and CAPS have very low metal-binding | *Phosphates and pyrophosphate are both substrates and inhibitors of different enzyme reactions (inhibition of carboxypeptidase, urease, various kinases, various dehydrogenases). [http://www.applichem.com/fileadmin/Broschueren/BioBuffer.pdf Applichem] | ||
constants and are therefore particularly suited to investigate metal-dependent enzymes (Good & Izawa 1972, Blanchard | |||
