7,287
edits
Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Knight:Purification of His-tagged proteins/Denaturing (view source)
Revision as of 11:09, 29 September 2006
, 29 September 2006→Lysis and column equilibration buffer (Qiagen buffer B)
(→Notes) |
|||
| Line 4: | Line 4: | ||
==Materials== | ==Materials== | ||
===Lysis and column equilibration buffer | ===Lysis and column equilibration buffer=== | ||
*8 M urea | *8 M urea | ||
*100 mM NaH<sub>2</sub>PO<sub>4</sub> | *100 mM NaH<sub>2</sub>PO<sub>4</sub> | ||
*10 mM Tris Cl | *10 mM Tris Cl | ||
*10 mM imidazole (recommended by Kathleen, 9/27/2007) | |||
*pH 8.0 | *pH 8.0 | ||
