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Lidstrom:Buffers: Difference between revisions
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→How to chose a buffer for enzyme assays
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* '''Pyrophosphate''' | * '''Pyrophosphate''' | ||
** Pyrophosphate buffers also precipitate polyvalent cations and have a tendency to form complexes. Like phosphate it is also a metabolite and may affect certain enzymes. | ** Pyrophosphate buffers also precipitate polyvalent cations and have a tendency to form complexes. Like phosphate it is also a metabolite and may affect certain enzymes. | ||
* '''Imidazole'' | * '''Imidazole''' | ||
** Imidazole has on occasions been used where phosphate is not acceptable, since both have similar buffering ranges. It is not generally a good alternative, since it is reactive, unstable, and also complexes with divalent metal ions. Mops or Bes might be a better alternative. | ** Imidazole has on occasions been used where phosphate is not acceptable, since both have similar buffering ranges. It is not generally a good alternative, since it is reactive, unstable, and also complexes with divalent metal ions. Mops or Bes might be a better alternative. | ||
* '''Borate''' | * '''Borate''' | ||
** Borate has the disadvantage of complexing with vic diols, which included many carbohydrates and the ribose moitey of nucleotides. It would be best avoided when assaying enzymes using NAD(P) or other nucleotides. | ** Borate has the disadvantage of complexing with vic diols, which included many carbohydrates and the ribose moitey of nucleotides. It would be best avoided when assaying enzymes using NAD(P) or other nucleotides. | ||
[[image:Buffers For Enzyme Assays.png|thumb|upright=3.0|center|buffers for enzyme assays, from [http://www.amazon.com/Enzyme-Assays-Practical-Approach-Series/dp/0199631425/ref=sr_1_1?ie=UTF8&qid=1383921457&sr=8-1&keywords=0199631425 ISBN 0-19-963142-5]]]] | |||
== Resources== | == Resources== | ||
