Lidstrom:Buffers: Difference between revisions

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== General Info ==
== General Info ==
== Does pH of a buffer depend on the concentration of buffer? ==
Blah blah<ref>[http://www.amazon.com/Enzyme-Assays-Practical-Approach-Series/dp/0199631425/ref=sr_1_1?ie=UTF8&qid=1383921457&sr=8-1&keywords=0199631425 ISBN 0-19-963142-5]</ref>
Citation: Book: [http://www.amazon.com/Enzyme-Assays-Practical-Approach-Series/dp/0199631425/ref=sr_1_1?ie=UTF8&qid=1383921457&sr=8-1&keywords=0199631425 ISBN 0-19-963142-5]
== Resources==
=== Book about buffers ===
* Helpful book chapter: [http://www.med.unc.edu/pharm/sondeklab/Lab%20Resources/protein_purification_handbooks/buffers_calbiochem.pdf protein purification handbook (Calbiochem)].  It is a little mammalian biased, but good.  
* Helpful book chapter: [http://www.med.unc.edu/pharm/sondeklab/Lab%20Resources/protein_purification_handbooks/buffers_calbiochem.pdf protein purification handbook (Calbiochem)].  It is a little mammalian biased, but good.  
**Table of Contents: (subset)
**Table of Contents: (subset)
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* '''Note [[User:Janet B. Matsen|Janet]] hasn't answered the original question!  In fact, this pdf only discussed sodium phosphate buffer.
* '''Note [[User:Janet B. Matsen|Janet]] hasn't answered the original question!  In fact, this pdf only discussed sodium phosphate buffer.


== How do method developer chose between sodium and potassium phospahte buffers? ==
== Q&A==
=== How do method developer chose between sodium and potassium phospahte buffers? ===
* Facts:
* Facts:
** The pKas for sodium and potassium phosphate salts are the same.
** The pKas for sodium and potassium phosphate salts are the same.
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