Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions

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Knight:Purification of His-tagged proteins/Denaturing (view source)

Revision as of 13:48, 24 September 2006

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==Overview==
==Overview==
"The high affinity of the Ni-NTA resins for 6xHis-tagged proteins or peptides is due to both the specificity of the interaction between histidine residues and immobilized nickel ions and to the strength with which these ions are held to the NTA resin." <cite>QiagenNTAManual</cite>
Denaturing purifications can often lead to better purity and yield.


==Materials==
==Materials==
Reshma P. Shetty
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