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BISC110/F13: Series 1 Lab 3 Tetrahymena Investigation: Difference between revisions
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BISC110/F13: Series 1 Lab 3 Tetrahymena Investigation (view source)
Revision as of 11:12, 16 September 2013
, 16 September 2013→PART III: Design your own experiment to investigate a variable that affects phagocytosis in Tetrahymena pyriformis
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'''Colchicine''' 25mM (10mg/ml) in water<BR> | '''Colchicine''' 25mM (10mg/ml) in water<BR> | ||
'''Nocodazole''' 7mM (2mg/ml) in DMSO<BR> | '''Nocodazole''' 7mM (2mg/ml) in DMSO<BR> | ||
'''India ink''' (Speedball, Product #3328, #3338, Statesville, NC), according to the manufacturer, contains (vol./vol.): water (75-85%), carbon black (7-9%), shellac (6-8%), ammonium hydroxide (1-2%), phenol (0.45%) and ethylene glycol (1.6%). The carbon black is dispersed as ~ 20nm carbon particles but it ranges, in the commercial grade used for India ink from 13-75nm or larger when aggregated. <BR | '''India ink''' (Speedball, Product #3328, #3338, Statesville, NC), according to the manufacturer, contains (vol./vol.): water (75-85%), carbon black (7-9%), shellac (6-8%), ammonium hydroxide (1-2%), phenol (0.45%) and ethylene glycol (1.6%). The carbon black is dispersed as ~ 20nm carbon particles but it ranges, in the commercial grade used for India ink from 13-75nm or larger when aggregated. <BR> | ||
'''Polybead Dyed Microspheres available:''' <BR> | '''Polybead Dyed Microspheres available:''' <BR> | ||
1.0µm Blue <BR> | 1.0µm Blue <BR> | ||
