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BISC110/F13: Series 1 Lab 3 Tetrahymena Investigation: Difference between revisions
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BISC110/F13: Series 1 Lab 3 Tetrahymena Investigation (view source)
Revision as of 08:44, 16 September 2013
, 16 September 2013→PART II: Ink and Tetrahymena Behavior
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#Put on a pair of gloves. | #Put on a pair of gloves. | ||
#To begin your experiment, add 50 μL of 1% ink solution and then add 50 μL of live ''Tetrahymena''. Mix gently and record the time. | #To begin your experiment, add 50 μL of 1% ink solution and then add 50 μL of live ''Tetrahymena''. Mix gently and record the time. | ||
#After 0, 5, and | #After 0, 5, and 10 minutes, add 20 μL of the ''Tetrahymena'' and ink solution to a clean labeled microcentrifuge tube. '''IN THE HOOD''', add 10 μL of 3% gluteraldehyde to your ''Tetrahymena'' and ink solution and wait for 1 minute. The glutaraldehyde will fix (kill) the cells, allowing you to observe the ''Tetrahymena'' in more detail. | ||
#'''IN THE HOOD''', add 20 μL of the resulting 30 μL mixture to a glass slide and place a cover slip on top. You can now remove the slide from the hood, but be sure to dispose of this slide in the hood when you are done. | #'''IN THE HOOD''', add 20 μL of the resulting 30 μL mixture to a glass slide and place a cover slip on top. You can now remove the slide from the hood, but be sure to dispose of this slide in the hood when you are done. | ||
#Using a digital camera, take photos of the ''Tetrahymena'' at the 0, 5, 10 | #Using a digital camera, take photos of the ''Tetrahymena'' at the 0, 5, and 10 minute timepoints. Please refer to the directions in[[Appendix H/F12 | Appendix H]] for detailed camera instructions. | ||
#Count the number of ink filled vacuoles in 10 individual ''Tetrahymena'' at each time point and record this information in your lab notebook. | #Count the number of ink filled vacuoles in 10 individual ''Tetrahymena'' at each time point and record this information in your lab notebook. | ||
#Using the micrometer, measure the size of these phagocytic vacuoles inside the ''Tetrahymena''. Measure at least 1 vacuole from at least 5 individual ''Tetrahymena''. Check with your instructor to be sure that you are measuring the correct structure. Did you see them form in the live "Tetrahymena"? Describe the process of phagocytosis in ''Tetrahymena'' in your lab notebook. | #Using the micrometer, measure the size of these phagocytic vacuoles inside the ''Tetrahymena''. Measure at least 1 vacuole from at least 5 individual ''Tetrahymena'' at the 5 minute timepoint and record this information in your lab notebook. Check with your instructor to be sure that you are measuring the correct structure. Did you see them form in the live "Tetrahymena"? Describe the process of phagocytosis in ''Tetrahymena'' in your lab notebook. | ||
=='''PART III: Design your own experiment to investigate a variable that affects phagocytosis in ''Tetrahymena pyriformis''== | =='''PART III: Design your own experiment to investigate a variable that affects phagocytosis in ''Tetrahymena pyriformis''== | ||
