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BISC209/F13: Lab3: Difference between revisions
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→SpectraMax 190:
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View of back of Spectra Max 190 <BR> | View of back of Spectra Max 190 <BR> | ||
Turn on the Spectra MAX 190 spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). The drawer will open. The drawer may, or may not, close on its own. If it doesn't close, please press the DRAWER button found on the Spectra MAX control panel (pictured below). It is important to keep the drawer closed as much as possible to prevent dust from entering the spectrophotometer. | Turn on the Spectra MAX 190 spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). The drawer will open. | ||
The drawer may, or may not, close on its own. If it doesn't close, please press the DRAWER button found on the Spectra MAX control panel (pictured below). It is important | |||
to keep the drawer closed as much as possible to prevent dust from entering the spectrophotometer. | |||
[[Image: Spectramax190.jpg]] | [[Image: Spectramax190.jpg]] | ||
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Position your 96 well plate into the tray drawer so that it fits securely in the holder and the well A1 is to the top left. Close it using the DRAWER button .<BR> | Position your 96 well plate into the tray drawer so that it fits securely in the holder and the well A1 is to the top left. Close it using the DRAWER button .<BR> | ||
Click: SETTING | Click: SETTING | ||
[[Image: Pro1a.jpg]]<BR> | |||
Set the wavelength to 590 for the BIOLOG Ecoplate. | |||
Check that Plate type is 96 well. | |||
Check that the Read area is the whole plate. | |||
[[Image: Pro2.jpg]] <BR> | [[Image: Pro2.jpg]] <BR> | ||
[[Image: Pro3.jpg]] <BR> | [[Image: Pro3.jpg]] <BR> | ||
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The data should be saved automatically in softmax pro, but please also save to our BISC209 folder on the desktop, labeling clearly with your group letter and the date. | The data should be saved automatically in softmax pro, but please also save to our BISC209 folder on the desktop, labeling clearly with your group letter and the date. | ||
Because the instrument computer is not networked, you will have to Save the data to a FLASH DRIVE. | Because the instrument computer is not networked, you will have to Save the data to a FLASH DRIVE. <BR> | ||
Export the labeled data to the thumb drive provided by your lab instructor. If you can export as an excel doc, do so. | Export the labeled data to the thumb drive provided by your lab instructor. If you can export as an excel doc, do so. | ||
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The Google Doc is pre-formatted with the calculations for these data. It includes the formulas to average replicate measurements each day and it will automatically subtract the background (readings in the water wells). There is a normalization for background that will be subtracted automatically (this threshold absorbance is 0.25 for each carbon source). We hope that including these pre-made calculations in the template will make your calculation of community metabolic diversity (CMD) and the data analysis of carbon source utilization patterns relatively uncomplicated. Please make sure you understand the EXCEL performed calculations. When you are sure that your data has been copied correctly into the appropriate template day, you will notice that the built in calculations will provide a final average absorbance reading for each substrate. | The Google Doc is pre-formatted with the calculations for these data. It includes the formulas to average replicate measurements each day and it will automatically subtract the background (readings in the water wells). There is a normalization for background that will be subtracted automatically (this threshold absorbance is 0.25 for each carbon source). We hope that including these pre-made calculations in the template will make your calculation of community metabolic diversity (CMD) and the data analysis of carbon source utilization patterns relatively uncomplicated. Please make sure you understand the EXCEL performed calculations. When you are sure that your data has been copied correctly into the appropriate template day, you will notice that the built in calculations will provide a final average absorbance reading for each substrate. | ||
Fill in the last column labled: '''SCORE 1 for wells with OD above 0.0'''. Once you fill in this final column, the template will calculate the total number of positive wells out of 31 for you and the SUM will appear in a cell below your data. This is the CMD for that day. The total will also appear on the last tab of the workbook (CMDtable&graph). | Fill in the last column labled: '''SCORE 1 for wells with OD above 0.0'''. Once you fill in this final column, the template will calculate the total number of positive wells out of 31 for you and the SUM will appear in a cell below your data. This is the CMD for that day. The total will also appear on the last tab of the workbook (CMDtable&graph). | ||
==Continue Isolation of Bacteria to Pure Culture as Examples of Soil Community Richness and Co-operative and Competitive Behavior== | ==Continue Isolation of Bacteria to Pure Culture as Examples of Soil Community Richness and Co-operative and Competitive Behavior== | ||
