3,811
edits
BISC209/F13: Lab2: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→RICHNESS OF A MICROBIAL SOIL COMMUNITY: Culturing a few Bacteria from a Soil Community to examine structural diversity
Tucker Crum (talk | contribs) |
|||
| Line 96: | Line 96: | ||
[[Image:Fig A-5.jpg]] <br> | [[Image:Fig A-5.jpg]] <br> | ||
Figure A-5: An example of growth 24-72 hours after isolation streaking a plate to obtain isolated colonies. | Figure A-5: An example of growth 24-72 hours after isolation streaking a plate to obtain isolated colonies.<BR> | ||
Over the next few weeks you will continue to sub-culture onto new plates, using your best isolation streak technique. Your goal is to continue to streak out ONE CFU until you are sure that all the bacterial growth in a colony comes from a single mother cell (pure culture). In subsequent labs you will make a bacterial smear and do a | |||
Over the next few weeks you will continue to sub-culture onto new plates, using your best isolation streak technique. Your goal is to continue to streak out ONE CFU until you are sure that all the bacterial growth in a colony comes from a single mother cell (pure culture). In subsequent labs you will make a bacterial smear and do a stain these genetically identical bacteria and you will perform other tests from freshly pure cultures to explore the physical and metabolic characteristics of this isolate. <BR><BR> | |||
Some bacteria often form tough leathery colonies, so transfer of these bacteria to new media to start a sub-culture is sometimes difficult. The powdery area may be spores, which would be interesting to visualize later. To isolate spore formers try to "break off" a piece of the colony with your sterile loop or with a sterile toothpick and transfer that whole piece of a colony onto zone one of the new plate. Then use your loop for streaking out the other zones. The tiny spores on the surface of the colony are likely to transfer to the next plate or tube when you work with it. (That's a good thing this time.) <BR><BR> | Some bacteria often form tough leathery colonies, so transfer of these bacteria to new media to start a sub-culture is sometimes difficult. The powdery area may be spores, which would be interesting to visualize later. To isolate spore formers try to "break off" a piece of the colony with your sterile loop or with a sterile toothpick and transfer that whole piece of a colony onto zone one of the new plate. Then use your loop for streaking out the other zones. The tiny spores on the surface of the colony are likely to transfer to the next plate or tube when you work with it. (That's a good thing this time.) <BR><BR> | ||
