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BISC209/F13: Lab2: Difference between revisions
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→RICHNESS OF A MICROBIAL SOIL COMMUNITY: Structural Diversity in Cultured Bacteria from a Soil Community
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<li>Check your plate for colonies daily and record your descriptions of the texture and shape of the colonies that appear. | <li>Check your plate for colonies daily and record your descriptions of the texture and shape of the colonies that appear. | ||
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[[Image:Fig A-2-2inch.jpg]] <br> | |||
Figure A-2: Two options for aseptic transfer into a plate. (a) Streaking a plate while holding the lid ajar. Note that the lid shields the agar from airborne contamination: (b) streaking a plate while holding the bottom of the plate. Note that the agar surface faces downward, thereby minimizing contamination from the air. | |||
[[Image:FigA-3.jpg]] <br> | |||
Figure A-3: Pattern for isolation streaking section 1 of a plate. | |||
[[Image:FigA-41inch.jpg]] <br> | |||
Figure A-4: Illustration of isolation streak technique section 1 to section 2. | |||
[[Image:Fig A-5.jpg]] <br> | |||
Figure A-5: An example of growth 24-72 hours after isolation streaking a plate to obtain isolated colonies. | |||
Over the next few weeks you will continue to sub-culture onto new plates, using your best isolation streak technique. Your goal is to continue to streak out ONE CFU until you are sure that all the bacterial growth in a colony comes from a single mother cell (pure culture). In subsequent labs you will make a bacterial smear and do a Gram stain of these genetically identical bacteria and you will perform other tests from freshly pure cultures to explore the physical and metabolic characteristics of this isolate. <BR><BR> | Over the next few weeks you will continue to sub-culture onto new plates, using your best isolation streak technique. Your goal is to continue to streak out ONE CFU until you are sure that all the bacterial growth in a colony comes from a single mother cell (pure culture). In subsequent labs you will make a bacterial smear and do a Gram stain of these genetically identical bacteria and you will perform other tests from freshly pure cultures to explore the physical and metabolic characteristics of this isolate. <BR><BR> | ||
