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BISC110/F13: Series 1 Lab 3 Tetrahymena Investigation: Difference between revisions
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BISC110/F13: Series 1 Lab 3 Tetrahymena Investigation (view source)
Revision as of 09:26, 11 June 2013
, 11 June 2013→PART III: Design your own experiment to investigate a variable that affects phagocytosis in Tetrahymena pyriformis
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In the next lab, you will have the opportunity to design your own experiment to study a factor that affects phagocytosis in ''Tetrahymena''. The cytoskeleton plays an important role in the architecture of the cell. Microfilaments (composed of actin) are located throughout the cell but they are found predominantly near the plasma membrane where they play a role in cell movement. Microtubules (composed of tubulin) form hollow tubes in the cell and serve as tracks along which materials are transported. They also play a key role in the organization of organelles and they form the mitotic spindle. Many research papers that explore phagocytosis propose that the cytoskeleton, particularly the actin containing elements of the cytoskeleton, are crucial to some or all of the parts of phagocytosis. There seems to be some conflicting evidence addressing the role of the tubulin containing part of the cytoskeleton. You could choose to explore either the role of microfilaments (actin) or microtubules (tubulin) in phagocytosis in ''Tetrahymena pyriformis''. Alternatively, you may choose to investigate whether particle size affects phagocytosis. <BR><BR> | In the next lab, you will have the opportunity to design your own experiment to study a factor that affects phagocytosis in ''Tetrahymena''. The cytoskeleton plays an important role in the architecture of the cell. Microfilaments (composed of actin) are located throughout the cell but they are found predominantly near the plasma membrane where they play a role in cell movement. Microtubules (composed of tubulin) form hollow tubes in the cell and serve as tracks along which materials are transported. They also play a key role in the organization of organelles and they form the mitotic spindle. Many research papers that explore phagocytosis propose that the cytoskeleton, particularly the actin containing elements of the cytoskeleton, are crucial to some or all of the parts of phagocytosis. There seems to be some conflicting evidence addressing the role of the tubulin containing part of the cytoskeleton. You could choose to explore either the role of microfilaments (actin) or microtubules (tubulin) in phagocytosis in ''Tetrahymena pyriformis''. Alternatively, you may choose to investigate whether particle size affects phagocytosis. <BR><BR> | ||
Before you leave today, in groups of 4, design your own experiment to answer one of several questions that may have come up from your observations or from your reading about ''Tetrahymena'' phagocytosis. Your instructor will explain your options and she/he will direct you to a research paper or other information related to the question that you and your group chooses to explore. The information given should help you figure out appropriate experimental conditions. Create a sharable document with your group including your names, experimental question, and experimental protocol (including showing how you will make all working solutions of reagents) and division of labor within the group. Make sure your protocol is approved by your instructor. <br><br> | Before you leave today, in groups of 4, design your own experiment to answer one of several questions that may have come up from your observations or from your reading about ''Tetrahymena'' phagocytosis. Your instructor will explain your options and she/he will direct you to a research paper or other information related to the question that you and your group chooses to explore. The information given should help you figure out appropriate experimental conditions. You should start by answering the Experimental Design Questions provided by your instructor. <br> | ||
After you have answered these questions and talked to your instructor, you can begin to work on your protocol. Create a sharable document with your group including your names, experimental question, and experimental protocol (including showing how you will make all working solutions of reagents) and division of labor within the group. Make sure your protocol is approved by your instructor. <br><br> | |||
'''Main Topic Choices:'''<BR> | '''Main Topic Choices:'''<BR> | ||
