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Protein Quantification Using ImageJ: Difference between revisions
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→Determining the concentration of protein in SDS-PAGE gel bands using ImageJ
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:- I think at this stage it's easiest to use key command "Ctrl+2" to continue numbering the subsequent lanes (less of a chance to mess up!) | :- I think at this stage it's easiest to use key command "Ctrl+2" to continue numbering the subsequent lanes (less of a chance to mess up!) | ||
: [[Image:imagejalllanes.jpg|200px]] | : [[Image:imagejalllanes.jpg|200px]] | ||
:- I do not know how to correct the inevitable mistake boxes that you are going to | :- I do not know how to correct the inevitable mistake boxes that you are going to make by accident and that cannot be undone...I'm sorry! Deleting them will cause useless white space where the rectangle was previously. My best advice if you find yourself in that predicament is to close the file, reopen it and start again. You will get really good at marking the lanes, I promise. If you are some kind of wizard who knows how to fix these mistake boxes without having to start over again, you should let me know! | ||
10. Once all lanes are defined, go to '''Analyze→Gels→Plot lanes''' (or use "Ctrl+3") to generate histograms of each lane | 10. Once all lanes are defined, go to '''Analyze→Gels→Plot lanes''' (or use "Ctrl+3") to generate histograms of each lane | ||
